Four-color charge-coupled device(CCD) light curves in the B, V, Rc and I c bands of the totaleclipsing binary system V1853 Orionis(V1853 Ori) are presented. By comparing our light curves with those published by previous investigators, it is determined that the O'Connell effect on the light curves has disappeared. By analyzing those multi-color light curves with the Wilson-Devinney code(W-D code),it is discovered that V1853 Ori is an A-type intermediate-contact binary with a degree of contact factor of f = 33.3%(3.7%) and a mass ratio of q = 0.1896(0.0013). Combining our 10 newly determined times of light minima together with others published in the literature, the period changes of the system are investigated. We found that the general trend of the observed minus calculated(O-C) curve shows a downward parabolic variation that corresponds to a long-term decrease in the orbital period with a rate of d P/dt =-1.96(0.46)×10~(-7) d yr~(-1). The long-term period decrease could be explained by mass transfer from the more-massive component to the less-massive one. By combining our photometric solutions with data from Gaia DR_2, absolute parameters were derived as M_1 = 1.20 M⊙, M_2 = 0.23 M⊙, R_1 = 1.36 R⊙and R_2 = 0.66 R⊙. The long-term period decrease and intermediate-contact configuration suggest that V1853 Ori will evolve into a high fill-out overcontact binary. 相似文献
Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.
